The Research Technologist 3 participates in design & execution of research lab projects. Conducts experimental tests & procedures & processes & analyzes data. Reviews & revises existing research methodologies & may develop new methodologies, mathematical &/or computer models for predicting data. Ensures overall lab compliance with all associated rules & regulations.
This role will also be involved in setting up and managing the lab, supporting brain tumor research to study chromatin and regulation of gene expression, and handling mice with brain tumors for studying tumor development and responses to intramural therapies.
The Brain Tumor Laboratory of Dr. Adam M. Sonabend in the Department of Neurological Surgery within the Northwestern University Feinberg School of Medicine requires a senior-level research technologist to manage daily operations within the lab. The lab’s mission is to perform translational research on brain tumors to personalize care for patients with a molecular rationale. The PI is a trained neurosurgeon and the lab is funded to study the role of Topoisomerase II in transcriptional regulation in glioblastomas and its therapeutic exploitation for personalized care.
The lab’s focus is on characterization of Topoisomerase II-derived transcriptional regulation on gliomas and its relation to modification of chromatin structure by this enzyme. This work will involve the use of cell lines and human specimens, biochemistry and molecular biology with an emphasis on chromatin immunoprecipitation and chromatin accessibility assays. Another interest of the lab is the identification of molecular features that predict glioma susceptibility to etoposide, a Topoisomerase II targeting poison, including DNA binding by this enzyme.
Please note: Incumbent will spend up to 75% of each shift walking and standing in research environments. The position involves working with de-identified human specimens, recombinant DNA and with biohazard-level 2 agents such as lenti-viruses.
Following overall research objectives, independently designs, plans, conducts and troubleshoots experiments, including:
Preparing media, culturing cancer cell lines, performing visibility studies using different drugs
Performing experiments to characterize the cell lines, clone and prepare plasmids for over-expression and silencing the genes on cancer cell lines
Performing animal experiments using mouse models, overseeing the breeding process for transgenic mice, monitoring tumor growth, analyzing the data and presenting it
Evaluates protocols and data obtained from groups of experiments and recommends changes to processes, procedures and techniques to improve quality and validity of results based upon overall project objectives.
Using an advanced level of skill, completes the following activities:
DNA, RNA, Protein isolation, purification and expression
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Decatenation assays to determine Topoisomerase 2 activity
Cell culture, cell isolation
Tissue fixation/processing: embedding, preparation of blocks and sectioning
Breeding and screening transgenic mice
Database management of colony
Treats mice with experimental therapies
Images tumor-bearing mice with luciferase-based technology
Performs analysis of survival
Observes experiments, collects, records, summarizes, analyzes and interprets research data.
Draws scientific conclusions.
Co-authors scientific papers.
Assists in overseeing lab compliance with IACUC protocols and adherence to institutional safety and responsible research standards
Negotiates purchases with vendors.
Maintains and updates portions of grant budgets.
Tracks and summarizes lab supply expenses.
Verifies accuracy of invoices.
Orders and/or maintains inventory of reagent, mice and/or lab supplies.
Trains and directs other lab EEs and/or students in research techniques and procedures.
Performs other duties as assigned.
Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience required; OR
Successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.
Minimum Competencies: (Skills, knowledge, and abilities.)
Molecular biology: DNA and RNA isolation from mammalian cell cultures and human tissues, CRISPR, PCR, RT-PCR, Q-PCR, cloning. Preparation of plasmids and viral particles for silencing and over-expression experiments.
Cell Culture-related skills: Establishment of cell preparations from mouse/human tumors to generate cell lines, mammalian cell cultures, antibiotic-selection, Transfection, immunostaining, evaluation of treatments for cancer in vitro, including cell viability with MTT Assay.
Protein Characterization: Isolation of proteins from whole cell and nuclear extracts, protein quantification, Western Blot, immune-precipitation, ELISA.
Animal experiment abilities: Perform animal experiments using mouse models, breed transgenic mouse strains, genotype mice, perform intracranial tumor cell injections, perform intratumoral drug delivery procedures, monitor tumor growth, follow mice for survival analysis, perform dissection of brain and other organs, analyze the data, & present it.
Demonstrated ability to follow detailed instructions and research protocols.
Demonstrated computer proficiency, including spreadsheets, database, and basic computations.
Meticulous attention to detail and adherence to ethical standards of research.
Precise and accurate record keeping.
Ability to work reliably, both independently and in a team environment.
Strong written and verbal communication skills.
Master in Biological Sciences is preferable.
2 years of experience working on brain tumor or translational cancer research.
Experience with whole-genome CRISPR/Cas9-based screening protocols.
Demonstrated experience in accounting and administrative skills relevant to the laboratory environment.
Microsoft Office (Word, Excel, PowerPoint).
Strong planning, organization and time management skills are necessary.
Experience with developing SOPs is a plus.
Preferred Competencies: (Skills, knowledge, and abilities)
Experience in drafting and maintaining compliance with IACUC protocols, as well as adherence to institutional safety and responsible research standards.
As per Northwestern University policy, this position requires a criminal background check. Successful applicants will need to submit to a criminal background check prior to employment.
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highl...y collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.