This individual will perform routine technical support in the laboratory to support research in identification of glycoproteins associated with HIV latent infection using glycoproteomics. Under close supervision this person is taught to perform and understand the principles for: routine specimen handling, accessioning and laboratory preparation following established procedures. The individual will prepare routine reagents or media, perform routine maintenance, and will be expected to gain a general understanding of laboratory operation to assist investigators as needed.
Duties and Responsibilities:
% of Time/Duties:
80% - Work closely with the project investigator, following laboratory procedures and testing protocols. This individual will provide technical supports in daily experimental work and complete routine laboratory tasks such as washing glassware, preparing work areas, prepares standard media, reagents, and chemical solutions used for testing purposes
20% - Perform chemistry protocols for proteomics study and data processing and analysis.
Equipment, Machine, or Tool Requirements: Ability to work with various laboratory techniques, tools and equipment.
Bachelor's degree in biological sciences, chemistry, or related field required.
Knowledge of good lab practices required. Some proteomic knowledge a plus.
Special Knowledge, Skills, or Abilities/Competencies:
Preferred skills and experience in tissue culture, flow cytometry, confocal microscopy, DNA purification, transfection, virus handling (expression/purification/titration/infection), enzyme-linked immunosorbent assay (ELISA), PCR, qPCR, RT-PCR, western blotting and general skills in molecular biology. Training will be provided.
Work Environment /While performing the duties of this job:
While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 65% of the time. Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus. The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform duties at laboratory work stations, gathering specimens, and loading equipment.
Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results. Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).
Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed. Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas. Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area. Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment. Research areas may include animal, human, human products, DNA, radiation protocols, and other bio-hazardous materials. Use of personal protective equipment may be advised or required.
May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory or on campus (specify)). May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes or Formalin cubes). In some areas work space is limited.
Staff may be required to complete annual competency review, and must insure compliance with Johns Hopkins Policy, Laboratory Policy & Procedure, Health, Safety & Environment regulations, and all applicable privacy & confidentiality laws/practices.
Many department laboratories operate 24/7 with schedules on day, evening, or night shift. Rotating schedules which may include weekend work and holiday assignment. (Overtime possible when authorized/needed to meet business and patient needs.
Previous experience in cell biology lab strongly preferred.
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