About the Stead Family Department of Pediatrics and University of Iowa Stead Family Children's Hospital
The Stead Family Department of Pediatrics is a national leader in pediatric medicine. The Department's mission is to provide outstanding care to the children of Iowa and beyond by being a leader in state-of-the-art clinical care to children, performing cutting-edge research to find new treatments and cures for childhood illnesses, and educating the next generation of pediatric health care providers. The Department comprises the medical and research staff of UI Stead Family Children's Hospital. UI Stead Family Children's Hospital is one of the nation's top-ranked pediatric care and research institutions, and Iowa's only comprehensive children's hospital. A new free-standing Children's Hospital is scheduled to open in 2017.
Shivanand R. Patil Cytogenetics and Molecular Laboratory
The Shivanand R. Patil Cytogenetics and Molecular Laboratory is located within the Division of Medical Genetics. The lab is a nationally accredited, CAP-inspected, CLIA certified facility that tests and analyzes subtle changes in genes and chromosomes to assist in the diagnosis of complex developmental problems in children and adults using state-of-the-art methods. The laboratory performs conventional chromosome analysis, fluorescence in situ hybridization (FISH), and chromosomal microarrays for a myriad of indications including cancer diagnosis/prognosis as well as both post and pre-natal genetics.
Within the Stead Family Department of Pediatrics, this position performs components of cytogenetic and molecular cytogenetic studies from biological specimen for diagnosis, prevention or treatment of disorders. This person will work to maintain and improve the testing environment, capabilities and quality of data in the Pediatric Cytogenetics Laboratory.
- Perform quantitative and qualitative analysis for all levels of testing complexity on laboratory clinical and research biological specimens such as blood, bone marrow, tissue and body fluids.
- Perform cell culture on varieties of tissues received for chromosome analysis according to established protocols.
- Process cultured cells and prepares slides according to laboratory protocol of chromosome spreads.
- Stain slides utilizing a variety of techniques including G-, C-, and Q-banding, NOR staining, SCE procedures and fluorescent in situ hybridization.
- Prepare microscope analysis of metaphases and prometaphase chromosomes for diagnostic purposes.
- Prepare digitized karyotypes using correct International System for Human Cytogenetic Nomenclature (ISCN).
- Perform DNA extraction.
- Perform DNA quality control and gel electrophoresis.
- Prepare and process DNA for molecular applications such as polymerase chain reaction (PCR).
- Process DNA for chromosomal microarray (CMA) applications including DNA fragmentation and labeling, hybridization to microarray, microarray washing, and microarray scanning.
- Process DNA for next generation sequencing (NGS) applications including library preparation, DNA sequencing, initial data analysis.
- Begin and ensure proper execution of analytic pipelines for both CMA and NGS applications.
- Initiate CMA and NGS data analysis pipelines by quality controlling raw data, placing raw data in appropriate file locations to begin analysis, starting analysis, checking quality control metrics as analysis proceeds to ensure proper execution.
- Maintain accurate and legible records of all lab results and procedures, and accurately interprets and records test results.
- Document all specimen information during accession process.
- Investigate quality assurance issues involving patient samples.
- Recognize basic problems including culture failure and contamination as well as DNA extraction failure, fragmented DNA, quality control metrics outside acceptable ranges, poor CMA and/or NGS data quality.
- Recommend corrective action for quality assurance.
- Seek technical direction from laboratory supervisor with specific instrumentation problems.
- Assist in reagent comparisons, evaluate results and recommend changes to reduce costs and improve efficiency.
- Perform daily operation and routine maintenance, calibration, and assist in troubleshooting of laboratory instruments such as incubators, microscopes, hoods, microarray wash stations and scanner, centrifuges, DNA sequencer, PCR thermocyclers, gel electrophoresis equipment.
- Participate in evaluation of equipment and recommend products for acquisition.
- Maintain inventory and orders supplies as needed.
- Comply with applicable regulatory and accreditation requirements of the laboratory section.
- Perform, record and review daily quality control associated with assigned testing.
- Document and report any variances.
- Adhere to quality standard procedures for high complexity testing facilities.
- Participate in the Quality Assurance program.
- Identify opportunities for improvement and make recommendations for resolution of identified problems/errors.
- Maintain quality control and quality assurance procedures in accordance with established policies and regulatory requirements; assemble quality control data for further analysis; recognize problems; and document issues; and initiate corrective actions after reporting problems.
- May assist with writing and revising laboratory procedures according to NCCLS guidelines.
- Assist in a positive and professional manner with bench training for other laboratory colleagues within the section.
- Participate in lab section meetings with others to discuss quality testing, workflow, efficiency strategies, problematic areas, and laboratory improvement.
*** Please note: Five (5) professional references will be requested and required at a later step in the recruitment process. ***