| The Buchon laboratory focuses on the impact of pathogens and the microbiota on body homeostasis. We use systemic infection as a model for septicemia, and the gut response to infection as a model for mucosal immunity. Genomic and genetic approaches allow us to characterize new pathways involved in both resistance and resilience/tolerance to infection. We are interested in the three-way dialogue between gut microbes, intestinal stem cells, and gut structure and function. The Buchon laboratory is part of the College of Agriculture and Live Sciences, the Department of Entomology and belongs to the Cornell Institute of Host-Microbe Interactions and Disease (CIHMID).|
The successful candidate will provide technical support in a research lab working on Drosophila immunity, intestinal physiology and stem cell biology. This includes:
- Maintaining the collection of Drosophila in the lab, and create new Drosophila lines by the use of transgenesis and genetics. Perform routine microbiology work, including handling of insect pathogens and gut microbiota.
- Performing research under the supervision of the laboratory Principal Investigator and collaborate on projects of other members of the laboratory.
- Assisting the principal investigator in all aspects of the daily and weekly management of the laboratory, including but not limited to purchases, budgets and maintenance of equipment. In parallel, the technician will be included in the intellectual life of the lab and is expected to perform some internet search and maintain laboratory records.
Laboratory techniques to be employed will include, but are not limited to molecular biology (DNA and RNA preparation, PCR/qPCR, sequencing, cloning), biochemistry (protein/western blots, biochemical dosage), manipulation and maintenance of bacterial cultures, animal stocks and cell lines, Drosophila genetics, dissection of live tissues, infection with insect pathogens, histology imaging and microscopy, and other techniques and procedures as necessary. The incumbent will help the Principal Investigator to insure that less experienced lab members adhere to laboratory safety protocol.
This is a full-time (39 hours per week) benefits eligible 6-month appointment with possible extension pending available funding and successful performance.
Bachelor's degree in Biological Sciences or equivalent combination of education and experience. Proficiency in basic computer programs (Microsoft Word, Excel etc.) is essential. Ability to follow instructions and protocols, have good interpersonal and communication skills, and a commitment to working as a member of a team. Must be willing and able to work with hazardous chemicals and biohazards. Ability to lift 20 pounds. Experience of one or more of basic molecular techniques, including DNA and RNA isolation and PCR, sterile technique, working with insects or microorganisms is an advantage.
University Job Title:Temp Serv Tech
Level:No Grade - Hourly
Pay Rate Type:Hourly
Contact Name:Tricia Reidy
Number of Openings:1
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