Salary is commensurate with education and experience
As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our Benefits page.
The University of Washington (UW) is proud to be one of the nation's premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement, artistic pursuits, and natural beauty.
The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.
A laboratory in the UW Department of Biochemistry currently has an outstanding opportunity for a RESEARCH SCIENTIST/ENGINEER 1for studies of membrane docking and fusion. This research project combines high-throughput saturation mutagenesis and deep sequencing approaches with structural analyses and biochemical reconstitution to understand the mechanisms of membrane docking and fusion.
The Research Scientist/Engineer 1 will:
Work with the PI to design and implement screening strategies to identify synthetic genetic interactions.
Design and construct saturation mutagenesis libraries using codon-level mutagenesis approaches.
Carry out and analyze synthetic interaction screens of the saturation libraries in Saccharomyces cerevisiae, using contemporary yeast genetic and deep sequencing approaches.
Analyze the results of the screens using the R statistical package and custom Python scripts, and perform structural modeling in PyMol or Chimera.
Clone and purify proteins from E. coli and/or S. cerevisiae using affinity and classical chromatography techniques.
Perform biochemical reconstitution experiments to test the functions of the proteins using fluorescence (FRET and anisotropy) techniques, and for structural characterization of the complexes by electron cryo microscopy and mass spectrometry.
Bachelor's degree in Chemistry or Biochemistry, and at least one year of laboratory research experience.
Experience with Saccharomyces genetics and protein purification are strongly preferred.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Other required qualifications include:
Practical understanding of principles of genetic analysis including suppression and epistasis.
Practical understanding of cloning strategies and tactics including but not limited to polycistronic vector design, inverse PCR, Gibson cloning, and cloning by homologous recombination (gap repair) in S. cerevisiae.
Experience with protein purification and liquid chromatography.
Experience using Python and R languages in a life science setting.
Strong organizational skills.
Ability to learn and integrate novel wet bench and in silico methods.
Excellent oral and written communication skills.
Ability and enthusiasm for working collaboratively as part of a team.
Experience with synthetic biology approaches to complex cloning problems.
Working knowledge of membrane traffic in eukaryotes.
Experience with PyMol or Chimera molecular modeling packages.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Criminal Conviction History, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select Apply to this position. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your My Jobs page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
CONDITION OF EMPLOYMENT:
Appointment of the successful candidate to this position may be made contingent upon a satisfactory outcome of a criminal conviction history check .
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergra...duate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.