Job Description Summary:
George Washington University’s Department of Microbiology, Immunology, and Tropical Medicine (MITM) conducts innovative, fundamental, basic and translational research for HIV/AIDS and neglected tropical diseases. MITM has a strong tradition of training and policy advocacy for these infectious and parasitic diseases, which initiate and perpetuate poverty and suffering worldwide. Additional information on MITM can be found at the following link: http://smhs.gwu.edu/microbiology/
MITM is searching for a full-time Research Assistant for Dr. Jones' laboratory. The Jones lab was established in April 2015, and focuses on studying the interaction between HIV-specific T-cells and NK cells with the viral reservoirs that persist in antiretroviral (ARV) treated individuals. In addition to describing this natural interaction we are actively developing methods to harness natural and engineered T cells to contribute to reservoir eradication. We are evaluating these approaches ex vivo using leukapheresis samples and in vivo using a humanized mouse model. We have a particular interest in translating promising approaches into the clinic. This position will aid the current researchers (Postdoctoral Scientist, Research Scientists and/or Lab Manager) in planning, executing, and analyzing immunology and virology experiments in the context of HIV-1 infection. The individual will conduct experiments under the direct supervision of a Senior Postdoc and will also receive mentoring and direct guidance from the Principal Investigator in order to advance their career in academic research.
Responsibilities include: Plans and schedules experiments consistent with the timeline and objectives of planned and ongoing research projects, including working with collaborators.
Performs mouse colony maintenance, dosing of mice with therapeutics in experimental protocols, assistance with surgical procedures and tissue extraction, and collection of behavioral and physical data.
Optimizes, develops, qualifies and executes the following: viral RNA extraction, HIV viral load analysis (qRT-PCR), ELISAs, plasma and PBMC isolation from whole blood, PBMC subpopulation isolation, flow cytometry, cell and tissue culture (including of HIV-infected human and animal tissues), DNA sequencing, and preparation of viral stocks.
Records and analyzes data, and summarizes results using basic statistical techniques
Performs general maintenance of good laboratory practice, compliance with Biosafety guidelines, maintenance of inspection records and generates SOPs and reports
Maintains and troubleshoots equipment
Works closely with students and Animal Resource Facility staff
Participates in intramural and extramural group meetings and presents data
Assist with the planning and execution of community outreach and educational events
Performs other work related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position.
Preferred Qualifications:Experience in immunological techniques including: ELISA and flow cytomety.
Experience with mouse colony maintenance and handling techniques.
Experience in molecular biology techniques, in particular viral RNA extraction and viral loads (qPCR), PCR, digital droplet PCR, and MiSeq sequencing will also be valued.
A BA/BS in related discipline is required, or an equivalent combination of education, training and experience.