ThisNIH funded laboratory investigates the molecular and cellular determinants ofalloantigen specific tolerance using murine models of cardiac and pancreaticislet transplantation. Specificinvestigations involve the determination of mechanisms responsible for remodelingof secondary lymphoid organ structure, and how those mechanisms drive T cellmigration, differentiation, and fate determination. The molecular focus of the investigations isdirected to chemokines, chemokine receptors, cytokines, sphingosine receptors, lymphotoxin,and stromal fibers. Specific cellularelements include regulatory suppressive T cells, effector T cells, vascular andlymphatic endothelial cells, stromal cells, and dendritic cell subsets. Techniques include heavy reliance on in vivo animalmodels, migration and trafficking assays, real time imaging and advancedmicroscopy, multicolor flow and sorting, and a variety of molecular techniquesfor proteins and nucleic acids.
WarrenKJ, Iwami D, Harris DG, Bromberg JS, Burrell BE. Vascular basement membraneproteins laminin alpha 4 and laminin alpha 5 differentially influence CD4+ Tcell lymph node trafficking and allograft fate. J. Clin. Invest. 2014;124:2204-2218.
XiongY, Ahmad S, Iwami D, Brinkman CC, Bromberg JS. T-bet regulates natural Tregafferent lymphatic migration and suppressive function. J. Immunol. 2016;196:2526-2540.
BrinkmanCC, Iwami D, Hritzko MK, Xiong, Y Ahmad, Bromberg JS. Treg engage lymphotoxinbeta receptor for afferent lymphatic transendothelial migration. NatureCommunications 2016; 7: 12021. DOI: 10.1038/ncommons12021