Life Science Research Professional 3 - 73858
Description Stanford University, located between San Francisco and San Jose in the heart of California's Silicon Valley, is one of the world's leading teaching and research universities. Since its opening in 1891, Stanford has been dedicated to finding solutions to big challenges and to preparing students for leadership in a complex world.
The Stanford Shared FACS Facility located at the Beckman Center is a fee-for-service center that is heavily used to support the cell sorting and analysis needs of the School of Medicine and the rest of the University by providing training, instrument access, operator support, and new method development.
The LSRP3 will focus on developing protocols for the use, operation, and maintenance of the DVS Mass Cytometer (CyTOF) as well as providing backup for other operations in the facility, including operation of flow cytometers for analysis and sorting for a wide variety of biological applications.
CyTOF instrument operation and training:
Operation, maintenance, and training on other Facility flow cytometers
- Become an expert user of, develop protocols for use, and independently operate a DVS CyTOF mass cytometer. Establish proper stream configuration of the instrument, sort instrument timing, sample compensation and population identification.
- Responsible for quality control procedures and weekly cleaning of the CyTOF (maching alignment, fluid replacement and removal, and sterilization and cleanup). Establish optimum running conditions;
- Develop training protocols and train FACS facility users in self-operation of the CyTOF;
- Provide users with data analysis for CyTOF generated data, using FlowJo, SPADE, and viSNE software.
* - Other duties may also be assigned
- Perform the functions of a staff LSRP in the facility, which includes:
- Independently operating cell analysis and sorting instruments in the Stanford Shared FACS Facility Service Center;
- Supporting and assisting self-operating users of the facility instruments;
- Training facility users in operation of facility instruments;
- Performing routine maintenance on facility equipment;
- Become familiar with all software used in the facility and assist facility users in learning to use this software;
- Understand and utilize common and unique biological applications, cell handling techniques, and reagents used by researchers and to assist them in designing and optimizing their FACS experiments;
- Work with research staff on implementation of updates to FACS Facility instruments and computer capabilities;
- Must be able to work a flexible schedule and to work in a BSL-2 environment to meet the needs of the facility.
Qualifications EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field and five years of related experience, or master's and two years of related experience.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- Comprehensive understanding of scientific principles.
- Expert level knowledge and skills in field of science related to research project.
- General computer skills, experience with databases and scientific applications, and ability to quickly learn and master computer programs.
- Strong analytical skills and excellent judgment.
- Ability to work under deadlines with general guidance is essential.
- Excellent organizational skills and demonstrated ability to complete detailed work accurately.
- Developing project management skills.
* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.
Location: School of Medicine
Job Code: 4953