RESEARCH SCIENTIST/ENGINEER 2
|Req #: ||139910 |
|Department: ||BIOCHEMISTRY |
|Job Location: || Health Sciences Center |
|Job Location Detail: ||Molecular Engineering and Sciences Building |
|Posting Date: ||11/29/2016 |
|Closing Info: || Open Until Filled |
|Salary: ||Salary is commensurate with education and experience. |
|Shift: ||1st |
|Notes: || As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our Benefits page. |
| The University of Washington (UW) is proud to be one of the nation's premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement, artistic pursuits, and natural beauty. |
The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.
The Department of Biochemistry currently has an outstanding opportunity for a RESEARCH SCIENTIST 2.
The UW Institute for Protein Design is seeking to hire a Research Scientist II whose duties will be to provide experimental support for research projects as part of the IPD Protein Production Facility. The Research Scientist/Engineer will be part of a small team focused on the expression, purification, and characterization of proteins for both internal investigators as well as external collaborators. Main duties will include development of methods for mammalian cell culture, transient transfection and transduction, stable cell line production, and harvesting of cell culture fluid for protein purification. Additional tasks will include protein purification and helping with general lab support as needed under supervision.
This position will require the performance of a variety of sterile tissue culture tasks including maintenance of human and animal derived cell cultures such as HEK293 and CHO cell lines, preparation of recombinant DNA for transfections, stable cell line production and maintenance, harvesting of culture media and cells for protein purification, protein purification, and analysis of protein samples from cell cultures by SDS-PAGE. A successful candidate will conduct mammalian cell tissue culture mainteance and DNA transfection / transduction for the Institute for Protein Design (IPD). Cell cultures will be mantained and used for protein expression testing and production of computationally designed proteins from recombinant DNA based vectors and other transduction systems.
Candidate must have familiarity with office productivity software (Word, Powerpoint, Excell) to prepare research reports for on the work performed. Candidate must be proficient with sterile laboratory maintenance, and familiar with BSL1-2 requirements. Candidate must have demonstrated professional competence and ability to to independently perform tissue culture assignments including protein production by transfection with recombinant DNA.
This position will facilitate the work of the Institute for Protein Design (IPD), adding capabilities to produce our designed proteins in a mammalian cell culture, within the Department of Biochemistry and the UW School of Medicine.
40% Maintenance of mammalian cell tissue cultures (e.g. human 293 cells),
30% Preparation of recombinant DNA for cell culture transfections (e.g. Qiagen Kits),
30% Affinity chromatography protein purification (e.g. IMAC and SEC) and analytics (e.g. SDS-PAGE)
Carry out established experimental protocols like plasmid preparation and extraction, transient transfection, and protein purification, under limited supervision.
Perform technical procedures such as gel electrophoresis and chromatography
Maintain mammalian cell cultures
Willingness to learn new techniques
Keep a detailed and organized electronic lab notebook
Participate in laboratory meetings and planning sessions
Prepare laboratory reagents
Service and maintain laboratory instruments as needed
Work well under limited supervision
May demonstrate and explain various laboratory techniques to laboratory personnel, students, and others
Perform related duties as required
| Bachelor's Degree in Molecular Biology / Biochemistry / Cell Biology or related Life Sciences. Equivalent experience may substitute for degree requirement. |
At least two years of tissue culture experience for production of secreted glycoproteins. The ideal candidate should have experience in producing viral glycoproteins (e.g. Flu hemagglutinin).
The position requires the ability to transport equipment along footpaths between multiple sites, and to lift equipment (~35lb) to/from a rolling cart. The IPD has facilities in two location on campus, 0.4 mi apart.
Experience with Windows and Mac operating systems and general computer use.
Strong and clear communication skills in English, both oral written, are required.
Ability to work with little supervision, through initiative and good judgment.
The position will be in a collaborative research environment with multiple stakeholders that will interact with this position
| Experience in sterile technique, mammalian cell culture, including diagnostics for contaminations by bacteria |
Experience preparing DNA for transfection into mammalian cell cultures
Experience working in BSL1-2 research facilities
Experience producing recombinant viral glycoproteins in mammalian cell culture
Experience in purification of recombinant viral glycoproteins from mammalian cell culture
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Criminal Conviction History, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select Apply to this position. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your My Jobs page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
|CONDITION OF EMPLOYMENT:|
Appointment of the successful candidate to this position may be made contingent upon a satisfactory outcome of a criminal conviction history check .