Life Science Research Professional 2 - 67270
The Stanford Cancer Institute (SCI) seeks a Life Science Research Professional 2 (LSRP) to perform more complex functions and activities involved in defined research projects, and independently conduct a portion of the research project(s). The LSRP will support a research program using high throughput methods to study the function and biogenesis of circular RNA, a recently discovered ubiquitous and uncharacterized component of eukaryotic gene expression. The laboratory utilizes genetics, biochemistry, molecular biology and bioinformatic and statistical approaches.
The LSRP will be expected to make original scientific contributions: developing novel protocols and procedures as well as interpreting and analyzing the results; participate in training and management of personnel; and lab management support, such as maintaining lab records and adhering to safety regulations.
Perform complex (lab and/or field) experiments for a major portion of the research project. Maintain detailed records of experiments and outcomes.
Interpret and analyze results, often where few guidelines exist. Based on prior results, contribute to development of new research protocols and procedures or adaptation of existing protocols.
Perform ongoing literature review to remain current with new procedures and related research; present findings for lab work group.
Contribute substantively to the preparation of papers for publication, especially in the results section. May contribute to publication of findings and present ongoing work and findings to colleagues at academic conferences.
Supervise (either formally or informally) staff or students as needed, including oversight and instruction on techniques, as well as consultation on project work. Serve as technical resource for other research staff.
Perform general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. May assist with purchasing laboratory equipment.
- Prior experience with the following techniques is desired:
- FACS sorting, experience with CRISPR gene editing protocols
- Fluorescence microscopy
- RNA biochemistry
- Computer programming, basic genome informatics
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field and three years of related experience, or Master's degree.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
Comprehensive understanding of scientific principles.
General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
Strong analytical skills and excellent judgment.
Ability to work under deadlines with general guidance is essential.
Excellent organizational skills and demonstrated ability to accurately complete detailed work.
- Ability to work independently in an accurate, efficient and technically rigorous manner are required.
- Excellent interpersonal, communication, and record-keeping abilities are imperative.
- Ability to perform the following techniques independently is required:
- Cell culture, including knowledge of sterile methods
- RNA-Seq library preparation, cell lysis procedures and RNA purification
- Cloning of DNA constructs for transfection in tissue culture, including construct and PCR primer design
Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
Must have correctible vision to perform duties of the job.
Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
Ability to lift heavy objects weighing up to 50 pounds.
Ability to work in a dusty, dirty, and odorous environment.
Position may require repetitive motion.
* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu
Location: School of Medicine
Job Code: 4952