University of California, San Francisco
Department of Obstetrics, Gynecology & Reproductive Sciences
This is a position in a highly dynamic research lab with primary focus on studying basic biology of human embryonic stem cells as well as human placenta in normal and pathological pregnancies. We are looking for an individual with extensive knowledge and experience in human stem cell biology, and advanced molecular biology techniques, including qRT-PCR, microarrays and sequencing. The Specialist should have experience in maintaining cultures of human stem cell lines, preferably embryonic, and have exquisite aseptic technique. Being able to perform standard cell culture related assays including cell viability assay, producing growth curves, preparing cells for FACS and performing immunohistochemistry is a must. The candidate will also perform other related duties. Strong organizational skills and immaculate record keeping are required. Knowledge of Microsoft Office including Power Point and Microsoft Excel are required. Knowledge of DNA/RNA purification, optimization of mammalian cell transfection, as well as PCR, qPCR, and Western blotting techniques.
Required qualifications include:
Proficiency with Western blotting techniques (cell lysis, preparation of SDS-PAGE gels, running gels, protein transfer from gel to membranes, use of chemiluminescence reporter systems). Excellent knowledge of and proficiency in aseptic technique, analytical lab methodology. Knowledge of cell culture system in which different substrates/medias are employed. Computer proficiency including MS Office, Photoshop, Endnote, photomicrography packages; ability to learn instrumentation software. Ability to work independently and in teams consisting of members with different degrees of knowledge or ability. Fine motor skills. Good oral and written communication skills in English. Good organizational skills. Knowledge of cellular physiology as related to biochemical pathways, growth requirements for cells in vitro, and cellular responses to varying conditions (e.g., different oxygen tensions). Knowledge of immunocytochemistry and other antibody-based techniques; light and fluorescence microscopy. Experience with preparing cell for FACS analysis. 2+ years of experience in working with human embryonic stem cells, including their maintenance, characterization and banking.
• Specialists appointed at the Assistant rank must possess a master’s degree (or equivalent degree) or five years of experience in the relevant specialization.
• Specialists appointed at the Associate rank must possess a master’s degree (or equivalent degree) or five to ten years of experience in the relevant specialization.
• Specialists appointed at the Full rank must possess a terminal degree (or equivalent degree) or ten or more years of experience in the relevant specialization.
Preferred qualifications include:
• Ability to perform FACS analysis.
• Experience in deriving new ESC lines.
• Post-graduate training in field.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary and rank will be commensurate with the applicants experience and training.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Please apply online at https://aprecruit.ucsf.edu/apply/JPF01021