Summary Description: The Department of Pathology and Cell Biology at the Columbia University Medical Center is recruiting an Associate Research Scientist in the laboratory of Dr. Kevin Gardner. The incumbent will study the basic mechanisms of epigenetic gene regulation in breast cancer. The position will have an integrated focus on fundamental mechanisms of chromatin-based transcriptional control and epigenetic regulation with an emphasis on the role of metabolic imbalance in regulating these pathways in breast cancer (Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19286-91; Nat Struct Mol Biol. 2010 Dec;17(12):1406-13; Nat Commun. 2012 Jan 17;3:633; Nat Commun. 2013;4:1449). Major goals include understanding the role of metabolism and transcriptional cross-talk between epigenetic co-regulators and hormone receptor-mediated signaling pathways in both enhancer function and promoter-targeted mechanisms of gene control. An essential component of this work will involve developing omic perspectives that will integrate next-generation sequencing with genomics, transcriptomics, epigenomics, proteomics and metabolomics using model systems and patient-derived samples. The projects will be supported by access to an established cohort of diverse breast cancer patient samples with extensive molecular and genomic characterization to aid in developing translation perspectives in gene-network and pathway discovery that will have practical implications for improving breast cancer diagnosis, treatment, and prevention. Other responsibilities will include: Managing the day to day operations of the laboratory. Maintenance of cell lines and carrying out immunoprecipitation assays. Ordering and maintaining inventory of laboratory supplies. Keeping track of orders and confirming billing. Setting up and performing experiments involving tissue culture, western blot, DNA, RNA, analysis. Maintaining records pertaining to the laboratory, including records of experimental results. Operating, maintaining and troubleshooting complex equipment (e.g. ABI 7300 Real-Time PCR system). Assisting in the training of new hires and other supporting staff. Enforcing safety regulations and helping to train students and fellows in laboratory procedures. Performing other related duties as necessary to the functioning of the laboratory.
Minimum Qualifications: Ph.D. or M.D. equivalent in areas of biochemistry, cancer biology, cell & molecular biology or related field.
Preferred Qualifications: A proven track record of publication in epigenomics, molecular biology, or stem cell biology with a combined strong interest and focus on basic mechanisms of biology and their translational implications for disease incidence and progression is preferred. Expertise in the performance and analysis of ChIP-seq is required. Familiarity with CRISPR/Cas9 technology and ATAC-seq analysis is highly desirable. Experience in murine model systems is strongly desired, but not essential. Familiarity with bioinformatic approaches and technologies in manipulating and interpreting deep sequencing data will be a significant advantage. We are looking for individual with a strong desire to work on fundamental research questions in cancer and chromatin biology in a highly collaborative and interdisciplinary research environment. Those candidates with a proven research experience and a history of an ability to independently drive a project forward in an interactive environment, with willingness to provide mentorship to junior lab members, is highly desirable. Prior experience in patient derived xenografts approaches and technology (PDX) would be very competitive.
As a member of the National Collegiate Athletic Association (NCAA) and the Council of Ivy Group Presidents (Ivy League), it is imperative that members of the Columbia University community, in all matters related to the intercollegiate athletics program, exhibit the highest professional standards and ethical behavior with regard to adherence to NCAA, Conference, University, and Department of Intercollegiate Athletics and Physical Education rules and regulations.
Columbia University is an Equal Opportunity/Affirmative Action employer.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It... seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.