The Geacintov Laboratory has a long track record of interdisciplinary research in mechanisms of DNA repair of chemically defined DNA lesions. These forms of DNA damage arise from the reactions of oxidatively generated reactive oxygen and nitrogen species formed during the inflammatory response with cellular DNA. Other kinds of DNA damage are due to reactions of metabolically activated forms of chemical carcinogens present in the environment with purines in DNA. If not repaired by cellular defense mechanisms, these DNA lesions give rise to mutations because of error-prone DNA replication, and ultimately to the development and promotion of tumorigenesis. In order to assess the impact of these forms of DNA damage on human health and disease, it is critically important to understand which lesions are repaired in cellular environments and which are not.
Our research is focused on designing and synthesizing structurally diverse DNA lesions that are incorporated site-specifically into plasmids that are transfected into human cells of different genetic backgrounds. By quantitatively analyzing the products of repair it is possible to evaluate the relative efficiencies of repair of oxidatively generated DNA lesions by competing cellular repair pathways of Base Excision Repair (BER) and Nucleotide Excision Repair (V. Shafirovich, et al. J. Biol Chem. 2016, 291, 5309). Examples of structurally defined DNA lesions that are substrates of human NER only, but not of BER, have been published recently (Rechkoblit et al. Nature Commun. 2017, 8, 965).
We are seeking to recruit a person with molecular biology training and with skills that include managing fibroblast cell cultures, preparing cell extracts, Western blots, and other routine biological and biochemical methods. Knowledge of CRISPR-Cas9 Gene Editing methods is desirable, but not essential. PhD in Chemistry or related field.
Interested candidates are invited to submit a letter of interest describing their skill sets and CVs with 2-3 names of references.
New York University is an Equal Opportunity Employer. New York University is committed to a policy of equal treatment and opportunity in every aspect of its hiring and promotion process without regard to race, color, creed, religion, sex, pregnancy or childbirth (or related medical condition), sexual orientation, partnership status, gender and/or gender identity or expression, marital, parental or familial status, caregiver status, national origin, ethnicity, alienage or citizenship status, veteran or military status, age, disability, predisposing genetic characteristics, domestic violence victim status, unemployment status, or any other legally protected basis. Women, racial and ethnic minorities, persons of minority sexual orientation or gender identity, individuals with disabilities, and veterans are encouraged to apply for vacant positions at all levels.
Founded in 1831, New York University is now one of the largest private universities in the United States. Of the more than 3,000 colleges and universities in America, New York University is one of only 60 member institutions of the distinguished Association of American Universities.