UCSD Layoff from Career Appointment: Apply by 10/6/17 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Eligible Disability Counseling and Consulting services (DCC) or Special Selection clients should contact their Vocational Rehabilitation Counselor for assistance.
Under supervision, the Lab Assistant performs standard repetitive laboratory and field experimental procedures that require technical, scientific knowledge and skills. The Lab Assistant will assist the supervisor and laboratory personnel in lab organization of mouse lines, specimens and lab procedures and protocols. Incumbent will also assist the PI and technicians in carrying out experiments, making routine analysis and preparing solutions. Technical functions include animal studies, and operating lab equipment.
Graduation from high school or a General Education Diploma and two years of laboratory experience or two years of college including courses in the natural, physical or social sciences and one year of laboratory experience; or an equivalent combination of education and experience; knowledge and abilities essential to the successful performance of the duties assigned to the position.
Skill, knowledge, and ability to perform standard and routine laboratory techniques such as, but not limited to: cell culture of lymphocytes, cell feeding, cell passaging, cell freezing, and cell restarting and harvesting for DNA/RNA extractions. Other tasks include cell preparation for experiments in 6 wells or 12 wells, DNA/RNA purification, nanodrop concentration analysis, and gel electrophoresis.
Knowledge of proper use and maintenance of laboratory equipment.
Knowledge of lab operations and rules for handling, storing and disposing of noninfectious waste as well as bio hazardous material.
Skill and knowledge of computer basic data base and word processing programs.
Ability to accurately weigh chemicals, prepare accurate dilutions and buffers.
Skills and knowledge of computer operations and programs to include Access, Excel and Word.
Ability to work with biological fluids, tissue cultures and very small specimens.
Experience in obtaining optical coherence tomography and fundus photography imagining in a clinical setting.
Experience in performing EBV transformation of lymphocytes.
Experience preparing and maintaining stem cell and tissue cultures such as, but not limited to: human, rodent, and rabbit ocular stem cells, including limbal stem cells, conjunctival stem cells, lend stem cells, retinal progenitor cells, retinal pigment epithelial cells, skin epithelial stem cells, fibroblasts, oral mucosal stem cells, and mesochymal stem cells derived from umbilical cord.
Employment is subject to a criminal background check.
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