The SGC-UNC, a center within the UNC Eshelman School of Pharmacy, is developing small molecule chemical probes for the historically understudied protein kinases. We are seeking a self-motivated and experienced research specialist to develop and run cell-based assays to support discovery of narrow spectrum kinase inhibitors. The position offers an exciting opportunity to generate new tools and reagents that will be made freely available to the research community. The ideal candidate must have a track record of impactful scientific contributions, outstanding communication skills, strong problem solving skills, and the ability to collaborate with scientists across other disciplines.
Education and Experience:
Bachelor’s degree in a discipline related to the area of assignment; or equivalent combination of training and experience. All degrees must be received from appropriately accredited institutions.
Ability to utilize molecular and cell biology techniques to construct, optimize, and run cell-based compound profiling assays for small molecule kinase inhibitors.
Develop, troubleshoot, and run cell-based assays using NanoBRET to determine the activity and selectivity of kinase inhibitors.
Ability to utilize liquid handling automation to set-up assay plates
Molecular Biology, including DNA/RNA isolation and quantitation, cloning, primer design, sequencing and quantitative PCR (Q- and QRT-PCR). Collaborate with team members to advance multiple chemical probe projects in parallel while meeting challenging timelines and metrics.
Write and maintain accurate Standard Operating Procedures for all assays. Capture and archive screening data from these assays in a robust, timely and readily-accessible format. Report screening data to team members and present updates at team meetings.
Monitor and update supply ordering and negotiate special volume-based pricing from vendors where appropriate.
Oversee laboratory equipment, schedule service calls, coordinate yearly service agreements and be the primary contact for service providers.
Assist in the familiarization of new lab members with selected laboratory responsibilities, accountabilities and training requirements; e.g., selection and ordering of reagents for project work; inventory management; proper handling, storage and disposal of reagents supplies and laboratory waste; record keeping using a lab notebook or electronic log of laboratory activities; health and safety training; use of personal protective equipment.
Preparation of Materials for Publication or Presentation
Demonstrated broad scientific and technical assay development expertise in the areas of: 1.Molecular Biology, including DNA/RNA isolation and quantitation, cloning, primer design, sequencing and quantitative PCR (Q- and QRT-PCR). 2.Cell biology, including culture of cell lines and primary cells, transfections and transductions, and cell-based reporter and functional assays.
Proficiency with quantitative enzyme assays, immunoprecipitation, ELISA, Western blot analysis, SDS page to complement the cellular compound profiling efforts.
Demonstrated ability to design and execute experiments, solve problems independently, troubleshoot methods, analyze and interpret data and present results.
Ability to work effectively in a multi-disciplinary team environment and adapt to changes in work priorities.
Strong written and oral communication skills
Experience developing and running screening assays to support chemistry projects is highly desirable
The University of North Carolina at Chapel Hill is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender, gender expression, gender identity, genetic information, race, national origin, religion, sex, sexual orientation, or status as a protected veteran.
About The University of North Carolina at Chapel Hill
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