Summary Description: Columbia University's Mortimer B. Zuckerman Mind Brain Behavior Institute (Zuckerman Institute) supports interdisciplinary neuroscience research and discovery by scientists and scholars across the university and promises to be the most comprehensive institute for brain science. Located in a state-of-the-art facility, world class researchers are brought together to transform our understanding of the brain and mind. We are conducting pioneering research into how the brain develops, performs, endures and recovers. From effective treatments for disorders like Alzheimer's and autism to advances in fields as fundamental as computer science, the arts, economics, law and social policy, the potential impact for humanity is staggering. The laboratories of Dr. Thomas Jessell and Dr. Richard Axel seek to hire a Staff Associate to lead the mouse transgenic group. Reporting to Dr. Axel and Dr. Jessell, the Staff Associate will work at the interface of cutting edge technology and high-impact research, driving innovative solutions for neuroscience. Having access to a variety of advanced facilities and technical resources, the Staff Associate will contribute to an intensely collaborative environment and enjoy the challenges of participating deeply in diverse scientific projects. We seek a highly motivated individual who is enthusiastic about a career in science. Responsibilities include, but are not limited to the following: - Performs microinjections for both generations of transgenic mice and targeted mice via pronuclear microinjection and blastocyst microinjection, respectively. Generation of ES cell-derived mice via blastocyst injection, Cas9/gRna Mediated Genome Engineering Cytoplasm injection for Cas9/sgRNA, Embryo & Sperm Cryopreservation and Revival, Genotype, IVF (In vitro Fertilization)- This role will require significant consultation with lab members. Therefore, the incumbent must work in partnership and collaboratively with the lab. - Must keep up with scientific literature, introducing new methodologies as they are developed. Maintains an understanding of the ever-changing field which has evolved with the large scale mouse Knockout projects (KOMP, EUCOMM, etc).- Provides individualized training and technical support, including assistance with experimental design and advice on sample preparations.- Under the guidance of the Principal Investigators, creates and outlines guidelines and protocols for usage; oversees systems reservation in iLab and compliance for system usage. Administers monthly billing and invoicing per lab usage; assist in yearly budget planning and in determining the recharge rate for each system.- Prepares IACUC experimental protocols and implement IACUC regulation.- Performs regular and ongoing system maintenance and troubleshooting; other duties as assigned by the Principal Investigators.
Minimum Qualifications: A Bachelor's degree in animal science, biological sciences, reproductive biology, or related field. At least six (6) years of prior experience in microinjection experience. Applicants must have a demonstrated track record in all aspects of transgenic mice production via DNA pronuclear injection. Prior experience performing gene targeting in mouse embryonic stem (ES) cells is preferred. Provides Technical Advice for the preparation of DNA for genotyping, microinjection or electroporation.
Preferred Qualifications: Advanced degree / Ph.D. in the biological sciences or related field is preferred. Ideal candidate may possess in-depth understanding and extensive hands-on experience in DNA Pronuclear Injection in mouse zygote, including mES Cell ?Mediated Gene Targeting. Preparation of budgets, and submission of transgenic core grants is preferred.
As a member of the National Collegiate Athletic Association (NCAA) and the Council of Ivy Group Presidents (Ivy League), it is imperative that members of the Columbia University community, in all matters related to the intercollegiate athletics program, exhibit the highest professional standards and ethical behavior with regard to adherence to NCAA, Conference, University, and Department of Intercollegiate Athletics and Physical Education rules and regulations.
Columbia University is an Equal Opportunity/Affirmative Action employer.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It... seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.